Effect of Plasmid pIJ1008 from Rhizobium leguminosarum on Symbiotic Function of Rhizobium meliloti
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چکیده
منابع مشابه
Symbiotic plasmid rearrangement in Rhizobium leguminosarum bv. viciae VF39SM.
A rearrangement between the symbiotic plasmid (pRleVF39d) and a nonsymbiotic plasmid (pRleVF39b) in Rhizobium leguminosarum bv. viciae VF39 was observed. The rearranged derivative showed the same plasmid profile as its parent strain, but hybridization to nod, fix, and nif genes indicated that most of the symbiotic genes were now present on a plasmid corresponding in size to pRleVF39b instead of...
متن کاملRhizobium gone native: unexpected plasmid stability of indigenous Rhizobium leguminosarum.
Lateral transfer of bacterial plasmids is thought to play an important role in microbial evolution and population dynamics. However, this assumption is based primarily on investigations of medically or agriculturally important bacterial species. To explore the role of lateral transfer in the evolution of bacterial systems not under intensive, human-mediated selection, we examined the associatio...
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The symbiotic plasmid (pSym) of Rhizobium leguminosarum bv. trifolii 4S5, which carries Tn5-mob, was successfully transferred into Agrobacterium tumefaciens A136 by using a conjugation method. The resulting transconjugants induced the development of ineffective nitrogen-fixing nodules on the roots of white clover seedlings. Depending on the manner in which the pSym was retained, the transconjug...
متن کاملThe lipo-oligosaccharidic symbiotic signals of Rhizobium meliloti.
288 time to identify the carbohydrate-bearing structures. Recent examples of glycoproteins originally detected with lectins that have now been identified are desmosomal glycoproteins [ 151 and integrins [ 161. Changes in the carbohydrate moieties of adhesion molecules can alter their function [ 17-19] and thus the lectin patterns may turn out to provide new insights into the changes in the adhe...
متن کاملGenetic transformation of Rhizobium leguminosarum by plasmid DNA.
We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No colonies that were doubly drug resistant were recovered in parallel control experiments.
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1984
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.47.4.876-878.1984